Papers and articles

List of project publications

Association of the human gut microbiota with vascular stiffness
Rafael R. C. Cuadrat, Tobias Goris, Anna Birukov, Fabian Eichelmann, Bruno G. N. Andrade, Corinna Bang, Andre Franke, Clemens Wittenbecher, Matthias B. Schulze
Peer-review article in Scientific Reports, 13, published 16 August 2023, Springer Nature
Open Access – Gold
https://doi.org/10.1038/s41598-023-40178-6

Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074
Suhui Ye, Patricia Magadán-Corpas, Álvaro Pérez-Valero, Claudio J. Villar, Felipe Lombó
Peer-review article in Microbial Cell Factories, in press, accepted 10 August 2023, Springer Nature
Open Access – Gold
https://doi.org/10.1186/s12934-023-02172-5

Technical upgrade of an open-source liquid handler to support bacterial colony screening
Irene Del Olmo Lianes, Pablo Yubero, Álvaro Gómez-Luengo, Juan Nogales, David R Espeso
Peer-review article in Frontiers in Bioengineering and Biotechnology, 11, 19 June 2023, Europe PMC
Open Access – Gold
https://doi.org/10.3389/fbioe.2023.1202836 

Synthetic Biology – High time to deliver?
Andrew D. Hanson, Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 16 June 2023, 12-6, American Chemical Society, ACS Publications
Open Access – Gold
https://doi.org/10.1021/acssynbio.3c00238

A highly selective cell-based fluorescent biosensor for genistein detection
Lucy Fang-I Chao, Dany Liu, Verena Siewers
Peer-review article in Engineering Microbiology, volume 3, issue 2, June 2023, online 04 February 2023, Elsevier B.V.
Open Access – Gold
https://doi.org/10.1016/j.engmic.2023.100078

 Pressure-dependent growth controls the 3D architecture of Pseudomonas putida microcolonies
Juhyun KimVíctor de LorenzoÁngel Goñi-Moreno
Peer-review article in Environmental Microbiology Reports, 25 May 2023,
Open Access – Gold
https://doi.org/10.1111/1758-2229.13182
https://pubmed.ncbi.nlm.nih.gov/37196337/

In vivo sampling of intracellular heterogeneity of Pseudomonas putida enables multi-objective optimization of genetic devices
Angeles Hueso-GilBelén Calles,  Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 16 June 2023, 12-6, first published: 17 May 2023, American Chemical Society, ACS Publications
Open Access – Gold
https://doi.org/10.1021/acssynbio.3c00009

Optimized De Novo Eriodictyol Biosynthesis in Streptomyces albidoflavus Using an Expansion of the Golden Standard Toolkit for Its Use in Actinomycetes
Patricia Magadán-Corpas, Suhui Ye, Álvaro Pérez-Valero, Patrick L McAlpine, Paula Valdés-Chiara, Jesús Torres-Bacete, Juan Nogales, Claudio J Villar, Felipe Lombó
Peer-review article in International Journal of Molecular Sciences, 24 (10), 17 May 2023, National Library of Medicine
Open Access – Gold
https://doi.org/10.3390/ijms24108879

Optimizing yeast for high-level production of kaempferol and quercetin
Musa Tartik, Juan Liu, Marta Tous Mohedano, Jiwei Mao, Yun Chen
Peer-review article in Microbial Cell Factories, 22, art.no.74, 20 April 2023, BioMed Central Ltd, part of Springer Nature
Open Access – Gold
https://doi.org/10.1186/s12934-023-02084-4

System metabolic engineering of Escherichia coli W for the production of 2-ketoisovalerate using unconventional feedstock
Darwin Carranza-Saavedra, Jesús Torres-Bacete, Blas Blázquez, Claudia Patricia Sánchez Henao, José Edgar Zapata Montoya, Juan Nogales
Peer-review article in Frontiers in Bioengineering and Biotechnology, 20 April 2023, Europe PMC
Open Access – Gold
https://doi.org/10.3389/fbioe.2023.1176445

New Insights on Metabolic Features of Bacillus subtilis Based on Multistrain Genome-Scale Metabolic Modeling
Blas Blázquez, David San Leon, Antonia Rojas, Marta Tortajada, Juan Nogales
Peer-review article in International Journal of Molecular Sciences, 24-8, 11 April 2023, Europe PMC
Open Access – Gold
https://doi.org/10.3390/ijms24087091

Water potential governs effector specificity of the transcriptional regulator XylR of Pseudomonas putida
Pavel DvořákTeca Calcagno GalvãoKatharina Pflüger-GrauAlice M. BanksVíctor de Lorenzo, Jose I. Jiménez
Peer-review article in Environmental Microbiology, volume 25, issue 5, first published: 22 January 2023, published by Applied Microbiology International and John Wiley & Sons Ltd.
Open Access – Gold
https://doi.org/10.1111/1462-2920.16342

Living architecture: metabolic applications for next-generation, selectively programmable bioreactors
Molly Hogle, Barbara Imhof, Waltraut Hoheneder, Rachel Armstrong, Ioannis Ieropoulos, Lauren Wallis, Jiseon You, Juan Nogales. Ed. Peter Droege
Chapter 21in book Urban and Regional Agriculture – Building Resilient Food Systems, published online 19 January 2023, Elsevier B.V
Open Access – Green
https://doi.org/10.1016/B978-0-12-820286-9.00004-2

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
Esteban Martínez-García, Sofía Fraile, Elena Algar, Tomás Aparicio, Elena Velázquez, Belén Calles, Huseyin Tas, Blas Blázquez, Bruno Martín, Clara Prieto, Lucas Sánchez-Sampedro, Morten H H Nørholm, Daniel C Volke, Nicolas T Wirth, Pavel Dvořák, Lorea Alejaldre, Lewis Grozinger, Matthew Crowther, Angel Goñi-Moreno, Pablo I Nikel, Juan Nogales, Víctor de Lorenzo
Peer-review article in Nucleic Acids Research, volume 51, issue D1, first published: 24 November 2022, published by Oxford University Press
Open Access – Gold
https://doi.org/10.1093/nar/gkac1059 

Innovation versus novelty in microbial systems
Víctor de Lorenzo
Peer-review article in Environmental Microbiology, volume 25, issue 1, first published: 06 November 2022, published by Applied Microbiology International and John Wiley & Sons Ltd.
Open Access – Gold
https://doi.org/10.1111/1462-2920.16278

Evaluation of double expression system for co-expression and co-immobilization of flavonoid glucosylation cascade
Agata Matera, Kinga Dulak, Sandra Sordon, Kacper Waśniewski, Ewa Huszcza, Jarosław Popłoński
Peer-review article in Applied Microbiology and Biotechnology, 106, published 05 November 2022, Springer Nature
Open Access – Gold
https://doi.org/10.1007/s00253-022-12259-5

A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
Dany Liu, Maria Sole Sica, Jiwei Mao, Lucy Fang-I Chao, Verena Siewers
Peer-review article in ACS Synthetic Biology, 22 September 2022, American Chemical Society, ACS Publications
Open Access – Gold
https://doi.org/10.1021/acssynbio.2c00111

Golden Standard: A complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria
Blas Blázquez, Jesús Torres-Bacete, David San Leon, Ryan Kniewel, Igor Martinez, Sandra Sordon, Aleksandra Wilczak, Sergio Salgado, Ewa Huszcza, Jarosław Popłoński, M. Auxiliadora Prieto, Juan Nogales
Non-peer reviewed article in bioRxiv, 20 September 2022, Cold Spring Harbour Laboratory, accepted with a change in title by Nucleic Acid Research in August 2023
Open Access – Gold
https://doi.org/10.1101/2022.09.20.508659

CRISPR/Cas9-enhanced Targetron Insertion for Delivery of Heterologous Sequences into the Genome of Gram-Negative Bacteria      
Elena Velázquez, Yamal Al-Ramahi, Víctor de Lorenzo
Peer-review article in Current Protocols, 2/9, first published: 06 September 2022, Current Protocols
Open Access – Gold
https://doi.org/10.1002/cpz1.532   

Novel flavonoid C-8 hydroxylase from Rhodotorula glutinis: identification, characterization and substrate scope
Kinga Dulak, Sandra Sordon, Agata Matera, Bartosz Kozak, Ewa Huszcza, Jarosław Popłoński
Peer-review article in Microbial Cell Factories, 21/175, published 29 August 2022, Springer Nature
Open Access – Gold
https://doi.org/10.1186/s12934-022-01899-x

Propagation of Recombinant Genes through Complex Microbiomes with Synthetic Mini-RP4 Plasmid Vectors
Tomás Aparicio, Jillian Silbert, Sherezade Cepeda, Víctor de Lorenzo
Peer-review article in BioDesign Research, 04 August 2022, SPJ-AAAS publisher
Open Access – Green
https://doi.org/10.34133/2022/9850305

The four Fs of the knowledge-based BioEconomy – A homage to Christian Patermann
Victor de Lorenzo
Peer-review article in EFB Bioeconomy Journal, November 2022, Vol. 2, online: 25 July 2022, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.bioeco.2022.100035

Environmental Galenics: large-scale fortification of extant microbiomes with engineered bioremediation agents
Victor de Lorenzo
Peer-review article in Philosophical transactions of the Royal Society B, 27 June 2022, The Royal Society Publishing
Open Access – Gold
https://doi.org/10.1098/rstb.2021.0395

Toward merging bottom-up and top-down model-based designing of synthetic microbial communities
David San León, Juan Nogales
Peer-review article in Current Opinion in Microbiology, Vol.69, October 2022, online: 25 Jun 2022, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.mib.2022.102169

Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes
Elena Velázquez, Beatriz Álvarez, Luis Ángel Fernández, Víctor de Lorenzo
Peer-review article in Microbial Biotechnology, 15/9, first published: 13 June 2022, Applied Microbiology International
Open Access – Gold
https://doi.org/10.1111/1751-7915.14098

Advances in constraint-based models: methods for improved predictive power based on resource allocation constraints
Eduard J. Kerkhoven
Peer-review article in Current Opinion in Microbiology, Vol.68, August 2022, first published: 09 June 2022, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.mib.2022.102168

Deep learning based kcat prediction enables improved enzyme constrained model reconstruction
Feiran Li, Le Yuan, Hongzhong Lu, Gang Li, Yu Chen, Martin K. M. Engqvist, Eduard J. Kerkhoven, Jens Nielsen
Peer-review article in Nature Catalysis, 5, published 16 June 2022, Nature Portfolio, part of Springer Nature
Open Access – Gold
https://doi.org/10.1038/s41929-022-00798-z

High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy
Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Peer-review article in Methods in Molecular Biology book series (MIMB, Vol.2479), online: 19 May 2022, Springer Science+Business Media, LLC, part of Springer Nature
Open Access – Gold
https://doi.org/10.1007/978-1-0716-2233-9_4

Standardization of regulatory nodes for engineering heterologous gene expression: a feasibility study
Pablo I. Nikel, Ilaria Benedetti, Nicolas T. Wirth, Víctor de Lorenzo, Belén Calles
Peer-review article in Microbial Biotechnology, 15/8, first published: 27 April 2022, Applied Microbiology International
Open Access – Gold
https://doi.org/10.1111/1751-7915.14063

Transformation of flavonoids by human gut microbiota – from in silico analyses to experimental confirmation
Tobias Goris, Annett Braune
Abstract Book – International Conference on Polyphenols, April 2022, 10th International Conference on Polyphenols and Health 2022, London
Open Access – Gold 
https://www.icph.info/media/emdbxqa2/icph-2022-abstract-book-with-front-cover-reduced-size.pdf

Discovery of Novel Tyrosine Ammonia Lyases for the Enzymatic Synthesis of p-Coumaric Acid
Yannik Brack, Chenghai Sun, Dong Yi, Uwe T. Bornscheuer
Peer-review article in ChemBioChem, 23/10, first published: 30 March 2022, Chemistry Europe
Open Access – Gold
https://doi.org/10.1002/cbic.202200062

Missing Links Between Gene Function and Physiology in Genomics
Julio Collado-Vides, Pascale Gaudet, Víctor de Lorenzo
Peer-review article in Frontiers in Physiology, 28 February 2022, Frontiers Media S.A.
Open Access – Gold
https://doi.org/10.3389/fphys.2022.815874

Versioning biological cells for trustworthy cell engineering
Jonathan Tellechea-Luzardo, Leanne Hobbs, Elena Velázquez, Lenka Pelechova, Simon Woods, Víctor de Lorenzo, Natalio Krasnogor
Peer-review article in Nature Communications, Vol. 13, Article number: 765 (2022), 09 February 2022
Open Access – Gold
https://doi.org/10.1038/s41467-022-28350-4

Antiproliferative and palliative activity of flavonoids in colorectal cancer
Javier Fernández, Blanca Silván, Rodrigo Entrialgo-Cadierno, Claudio J. Villar, Raffaele Capasso, José Antonio Uranga, Felipe Lombó, Raquel Abalo
Peer-review article in Biomedicine & Pharmacotherapy, 143, Nov 2021, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.biopha.2021.112241

Recent advances in model-assisted metabolic engineering
Steinn Gudmundsson, Juan Nogales
Peer-review article in Current Opinion in Systems Biology, Vol. 28, first published 12 October 2021, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.coisb.2021.100392

ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdán, Gyorgyi Ferenc, Csaba Pál, Luis Ángel Fernández, Víctor de Lorenzo
Peer-review article in Communications Biology, Vol. 4, Article number: 1169 (2021), 07 October 2021
Open Access – Green
https://doi.org/10.1038/s42003-021-02702-0

Targetron-Assisted Delivery of Exogenous DNA Sequences into Pseudomonas putida through CRISPR-aided Counterselection
Elena Velázquez, Yamal Al-Ramahi, Jonathan Tellechea-Luzardo, Natalio Krasnogor, Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 2 October 2021, ACS publications
Open Access – Green
https://doi.org/10.1021/acssynbio.1c00199

Standardization of inducer-activated broad host range expression modules: debugging and refactoring an alkane-responsive AlkS/PalkB device
Alejandro Arce-Rodríguez, Alejandro Arce-Rodríguez, Ilaria Benedetti, José Manuel Borrero-de Acuña, Rafael Silva-Rocha, Rafael Silva-Rocha, Víctor de Lorenzo
Peer-review article in Synthetic Biology, 6/1, 17 September 2021, Oxford Academic
Open Access – Gold
https://doi.org/10.1093/synbio/ysab030

Automated design and implementation of a NOR gate in Pseudomonas putida.
Huseyin Tas, Lewis Grozinger, Angel Goñi-Moreno, Víctor de Lorenzo
Peer-review article in Synthetic Biology, 6/1, 01 September 2021, Oxford Academic
Open Access – Gold 
https://doi.org/10.1093/synbio/ysab024

Terpenoids and Polyphenols as Natural Antioxidant Agents in Food Preservation
Ignacio Gutiérrez-Del-Río, Sara López-Ibáñez, Patricia Magadán-Corpas, Luis Fernández-Calleja, Álvaro Pérez-Valero, Mateo Tuñón-Granda, Elisa M. Miguélez, Claudio J. Villar, Felipe Lombó
Peer-review article in Antioxidants, 10/8, 8 August 2021, MDPI
Open Access – Gold
https://doi.org/10.3390/antiox10081264

Flavonoid-Modifying Capabilities of the Human Gut Microbiome—An In Silico Study
Tobias Goris, Rafael R.C. Cuadrat, Annett Braune
Peer-review article in Nutrients, 2021, 13/8, 03 August 2021, MDPI
Open Access – Gold
https://doi.org/10.3390/nu13082688

Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies
Sofía Fraile, María Briones, Mónica Revenga-Parra, Víctor de Lorenzo, Encarnación Lorenzo, Esteban Martínez‐García
Peer-review article in ACS Synthetic Biology, 10/8, first published 02 August 2021, ACS Publications
Open Access – Gold
https://doi.org/10.1021/acssynbio.1c00227

Picking the right metaphors for addressing microbial systems: economic theory helps understanding biological complexity
Juhyun Kim, Rafael Silva-Rocha, Víctor de Lorenzo
Peer-review article in International Microbiology, 24, first published 16 July 2021, Springer Link
Open Access – Gold
https://doi.org/10.1007/s10123-021-00194-w

De novo biosynthesis of garbanzol and fustin in Streptomyces albus based on a potential flavanone 3-hydroxylase with 2-hydroxylase side activity
Laura Marín, Ignacio Gutiérrez-Del-Río, Claudió Jesus Villar, Felipe Lombó
Peer-review article in Microbial Biotechnology, 14/5, first published 03 July 2021, sfam
Open Access – Gold
https://doi.org/10.1111/1751-7915.13874

Recent trends in biocatalysis
Dong Yi, Thomas Bayer, Christoffel P.S. Badenhorst, Shuke Wu, Mark Doerr, Matthias Höhne, Uwe T. Bornscheuer
Peer-review article in Chemical Society Reviews, 50/14, first published 18 June 2021
Open Access – Gold
https://doi.org/10.1039/D0CS01575J

Discovery of Novel Bacterial Chalcone Isomerases by a Sequence-Structure-Function-Evolution Strategy for Enzymatic Synthesis of (S)-Flavanones
Hannes Meinert, Dong Yi, Bastian Zirpel, Eva Schuiten, Torsten Geißler, Egon Gross, Stephan I. Brückner, Beate Hartmann, Carsten Röttger, Jakob P. Ley, Uwe T. Bornscheuer
Peer-review article in Angewandte Chemie International Edition, 60/31, first published 15 June 2021, Wiley Online Library
Open Access – Gold
https://doi.org/10.1002/anie.202107182

An updated structural model of the A domain of the Pseudomonas putida XylR regulator poses an atypical interplay with aromatic effectors
Pavel Dvořák, Carlos Alvarez-Carreño, Sergio Ciordia, Alberto Paradela, Víctor de Lorenzo
Peer-review article in Environmental Microbiology, 23/8, first published 07 June 2021, sfam
Open Access – Gold
https://doi.org/10.1111/1462-2920.15628

Reconstruction of a Genome-Scale Metabolic Model of Streptomyces albus J1074: Improved Engineering Strategies in Natural Product Synthesis
Cheewin Kittikunapong, Suhui Ye, Patricia Magadán-Corpas, Álvaro Pérez-Valero, Claudio J. Villar, Felipe Lombó, Eduard J. Kerkhoven
Peer-review article in Metabolites, 11/5, 11 May 2021, MDPI
Open Access – Gold
https://doi.org/10.3390/metabo11050304

A portable library of phosphate-depletion based synthetic promoters for customable and automata control of gene expression in bacteria
Jesús Torres-Bacete, José Luís García, Juan Nogales
Peer-review article in Microbial Biotechnology, 14/6, first published 30 March 2021, sfam
Open Access – Gold
https://doi.org/10.1111/1751-7915.13808

Transcriptional control of 2,4-dinitrotoluene degradation in Burkholderia sp. R34 bears a regulatory patch that eases pathway evolution
Danilo Pérez-Pantoja, Pablo I. Nikel, Max Chavarría, Víctor de Lorenzo
Peer-review article in Environmental Microbiology, 23/5, first published 18 March 2021, sfam
Open Access – Green
https://doi.org/10.1111/1462-2920.15472

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2
Juhyun Kim, Angel Goñi-Moreno, Víctor de Lorenzo
Peer-review article in mBio, published online 23 February 2021, American Society for Microbiology
Open Access – Green
https://doi.org/10.1128/mbio.03685-20

Low CyaA expression and anticooperative binding of cAMP to CRP frames the scope of the cognate regulon of Pseudomonas putida
Alejandro ArceRodríguez, Pablo I. Nikel, Belén Calles, Max Chavarría, Raúl Platero, Tino Krell, Victor de Lorenzo
Peer-review article in Environmental Microbiology, Early View, first published: 8 February 2021. Society for Applied Microbiology and John Wiley & Sons Ltd., UK.
Open Access – Green
https://doi.org/10.1111/1462-2920.15422

For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!
Víctor de Lorenzo, Natalio Krasnogor, Markus Schmidt
Peer-review article in New Biotechnology, 60, published: 25 January 2021, Elsevier BV
Open Access – Green
https://doi.org/10.1016/j.nbt.2020.08.004

Contextual dependencies expand the re-usability of genetic inverters
Huseyin Tas, Lewis Grozinger, Ruud Stoof, Victor de Lorenzo, Ángel Goñi-Moreno
Peer-review article in Nature Communications, 12/1, 2021, published: 13 January 2021, Springer Nature
Open Access – Green
https://doi.org/10.1038/s41467-020-20656-5

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress
Pablo I. Nikel, Tobias Fuhrer, Max Chavarría, Alberto Sánchez-Pascuala, Uwe Sauer & Victor de Lorenzo
Peer-review article in The ISME Journal, 2021, published: 11 January 2021, Springer Nature
Open Access – Gold
https://doi.org/10.1038/s41396-020-00884-9

The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA-LexA interplay
Özlem Akkaya, Tomás Aparicio, Danilo PérezPantoja, Víctor de Lorenzo
Peer-review article in Environmental Microbiology, Early view, first published: 3 January 2021, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Green
https://doi.org/10.1111/1462-2920.15384

In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
Beatriz Álvarez, Mario Mencía, Víctor de Lorenzo, Luis Ángel Fernández
Peer-review article in Nature Communications, 11 /1, 2020, published: 22 December 2020, Springer Nature
Open Access – Green
https://doi.org/10.1038/s41467-020-20230-z

A Standardized Inverter Package Borne by Broad Host Range Plasmids for Genetic Circuit Design in Gram-Negative Bacteria
Huseyin Tas, Ángel Goñi-Moreno, Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 10/1, 2020, published: 18 December 2020. American Chemical Society
Open Access – Green
https://doi.org/10.1021/acssynbio.0c00529

Concomitant prediction of environmental fate and toxicity of chemical compounds
Juan Antonio Garcia-Martin, Max Chavarría, Victor de Lorenzo, Florencio Pazos
Peer-review article in Biology Methods and Protocols, 5/1, 2020, published: 13 November 2020, Oxford Academic
Open Access – Gold
https://doi.org/10.1093/biomethods/bpaa025

Repositioning microbial biotechnology against COVID19: the case of microbial production of flavonoids
Tobias Goris, Álvaro PérezValero, Igor Martínez, Dong Yi, Luis FernándezCalleja, David San León, Uwe T. Bornscheuer, Patricia MagadánCorpas, Felipe Lombó, Juan Nogales
Peer-review article in Microbial Biotechnology, 14/ 1, 2021, first published: 13 October 2020, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Gold
https://doi.org/10.1111/1751-7915.13675

An automated DIY framework for experimental evolution of Pseudomonas putida
David R. Espeso, Pavel Dvořák, Tomás Aparicio, Víctor de Lorenzo
Peer-review article in Microbial Biotechnology, Online Version of Record before inclusion in an issue, first published: 13 October 2020, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Gold
https://doi.org/10.1111/1751-7915.13678

Quantitative assessment of morphological traits of planktonic bacterial aggregates
David R. Espeso, Esteban Martínez-García, Víctor de Lorenzo
Peer-review article in Water Research, Volume 188, 1 January 2021, first published: 28 September 2020, Elsevier BV
Open Access – Green
https://doi.org/10.1016/j.watres.2020.116468

Metabolic modelling approaches for describing and engineering microbial communities
Beatriz García-Jiménez, Jesús Torres-Bacete, Juan Nogales
Peer-review article in Computational and Structural Biotechnology Journal, volume 19, 2021, 15 December 2020, first published 23 September 2020, Elsevier B.V.
Open Access – Gold
https://doi.org/10.1016/j.csbj.2020.12.003

Surface display of designer protein scaffolds on genome-reduced strains of Pseudomonas putida
Pavel Dvořák, Edward A. Bayer, Víctor de Lorenzo
Research article in ACS Synthetic Biology, 2020, 9/10, 2 September 2020, American Chemical Society
Open Access – Gold
https://doi.org/10.1021/acssynbio.0c00276

Naked Bacterium: Emerging Properties of a Surfome-Streamlined Pseudomonas putida Strain
Esteban Martínez-García, Sofía Fraile, David Rodríguez Espeso, Davide Vecchietti, Giovanni Bertoni, Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 2020, 9/9, 30 July 2020, American Chemical Society
Open Access – Green
https://doi.org/10.1021/acssynbio.0c00272

A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria
Gonzalo Durante-Rodríguez, Belén Calles, Víctor de Lorenzo, Pablo Nikel
Peer-review article in BIO-PROTOCOL, 10/14, July 2020. Bio-Protocol, LLC
Open Access – Gold
https://doi.org/10.21769/bioprotoc.3688

Environmental Performance of Pseudomonas putida with a Uracylated Genome
Elena Algar, Yamal AlRamahi, Víctor de Lorenzo, Esteban MartínezGarcía
Peer-review article in ChemBioChem, 21/22, 2020, first published: 29 June 2020, Wiley Online Library
Open Access – Gold
https://doi.org/10.1002/cbic.202000330

The Wsp intermembrane complex mediates metabolic control of the swimattach decision of Pseudomonas putida
Ángeles HuesoGil, Belén Calles, Víctor de Lorenzo
Peer-review article in Environmental Microbiology, Volume 22, Issue 8, August 2020, first published: 9 June 2020, Society for Applied Microbiology and John Wiley & Sons Ltd.
Open Access – Green
https://doi.org/10.1111/1462-2920.15126

Exploiting geometric similarity for statistical quantification of fluorescence spatial patterns in bacterial colonies
David R. Espeso, Elena Algar, Esteban Martínez-García, Víctor de Lorenzo
Peer-review article in BMC Bioinformatics, 21, 3 June 2020, Springer Nature
Open Access – Green OA
https://doi.org/10.1186/s12859-020-3490-1

Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesion with Antagonistic Competitor Cells
See-Yeun Ting, Esteban Martínez-García, Shuo Huang, Savannah K. Bertolli, Katherine A. Kelly, Kevin J. Cutler, Elizabeth D. Su, Hui Zhi, Qing Tang, Matthew C. Radey, Manuela Raffatellu, S. Brook Peterson, Víctor de Lorenzo, Joseph D. Mougou
Peer-review article in Cell Host & Microbe, 28/2, August 2020, first published: 28 May 2020, Elsevier BV
Open Access – Green
https://doi.org/10.1016/j.chom.2020.05.006

Biotransformation of Dxylose to Dxylonate coupled to mediumchainlength polyhydroxyalkanoate production in cellobiosegrown Pseudomonas putida EM42
Pavel Dvořák, Jozef Kováč, Víctor de Lorenzo
Peer-review article in Microbial Biotechnology, 13/4, July 2020, first published: 3 May 2020, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access -Green
https://doi.org/10.1111/1751-7915.13574

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic
A. David PáezEspino, Pablo I. Nikel, Max Chavarría, Víctor de Lorenzo
Peer-review article in Environmental Microbiology, 22/ 6, June 2020, first published: 23 March 2020, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Green
https://doi.org/10.1111/1462-2920.14991

Multifunctional SEVA shuttle vectors for actinomycetes and Gramnegative bacteria
Coral GarcíaGutiérrez, Tomás Aparicio, Lucía TorresSánchez, Esteban MartínezGarcía, Víctor Lorenzo, Claudio J. Villar, Felipe Lombó
Peer-review article in MicrobiologyOpen, Volume 9, Issue 6, June 2020, first published: 14 March 2020, John Wiley & Sons Ltd
Open Access – Gold
https://doi.org/10.1002/mbo3.1024

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering.
Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Peer-review article in iScience, 23/ 3, March 2020, first published: 26 February 2020, Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.isci.2020.100946

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts
Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo
Peer-review article in Nucleic Acids Research, 48/ D1, 8 January 2020, first published: 19 November 2019. Oxford University Press
Open Access -Gold
https://doi.org/10.1093/nar/gkz1024

Digitalizing heterologous gene expression in Gramnegative bacteria with a portable ON/OFF module
Belén Calles, Ángel GoñiMoreno, Víctor Lorenzo
Peer-review article in Molecular Systems Biology, 15/ 12, 19 December 2019, EMBO
Open Access – Gold
https://doi.org/10.15252/msb.20188777

Multiple-Site Diversification of Regulatory Sequences Enables Interspecies Operability of Genetic Devices
Angeles Hueso-Gil, Ákos Nyerges, Csaba Pál, Belén Calles, Víctor de Lorenzo
Peer-review article in ACS Synthetic Biology, 9/1, 03 December 2019, American Chemical Society
Open Access – Gold
https://doi.org/10.1021/acssynbio.9b00375

Rewiring carbon metabolism in yeast for high level production of aromatic chemicals
Quanli Liu, Tao Yu, Xiaowei Li, Yu Chen, Kate Campbell, Jens Nielsen, Yun Chen
Peer-review article in Nature Communications, 10/1, 31 October 2019,
Open Access – Gold
https://doi.org/10.1038/s41467-019-12961-5

Highquality genomescale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities
Juan Nogales, Joshua Mueller, Steinn Gudmundsson, Francisco J. Canalejo, Estrella Duque, Jonathan Monk, Adam M. Feist, Juan Luis Ramos, Wei Niu, Bernhard O. Palsson
Peer-review article in Environmental Microbiology, 22/1 January 2020, first published: 27 October 2019, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Gold
https://doi.org/10.1111/1462-2920.14843

Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene
Tomas Aparicio, Akos Nyerges, István Nagy, Csaba Pal, Esteban MartínezGarcía, Víctor Lorenzo
Peer-review article in Environmental Microbiology, 22 /1, January 2020, first published: 09 October 2019, Society for Applied Microbiology and John Wiley & Sons Ltd
Open Access – Gold
https://doi.org/10.1111/1462-2920.14814

A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria
Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Peer-review article in Horizontal Gene Transfer, first online: 05 October 2019, Springer Nature
Open Access – Green
https://doi.org/10.1007/978-1-4939-9877-7_27

Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery
Elena Velázquez, Víctor de Lorenzo, Yamal Al-Ramahi
Peer-review article in ACS Synthetic Biology, 8/9, 16 August 2019, American Chemical Society
Open Access – Green
https://doi.org/10.1021/acssynbio.9b00293

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida
Alberto Sánchez-Pascuala, Lorena Fernández-Cabezón, Víctor de Lorenzo, Pablo I. Nikel
Peer-review article in Metabolic Engineering, Volume 54, July 2019, Elsevier BV
Open Access – Green
https://doi.org/10.1016/j.ymben.2019.04.005

Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
David R. Espeso, Esteban Martínez-García, Víctor de Lorenzo
Peer-review article in Bio-protocol, 9/10, 20 May 2019,
Open Access – Gold
https://doi.org/10.21769/bioprotoc.3238

Pseudomonas putida in the quest of programmable chemistry
Esteban Martínez-García, Víctor de Lorenzo
Peer-review article in Current Opinion in Biotechnology, Volume 59, online 30 April 2019,
Elsevier BV
Open Access – Gold
https://doi.org/10.1016/j.copbio.2019.03.012